METHOD FOR REMOVING LIPOPOLYSACCHARIDES(LPS) FROM DNA OR RNA IN CELL

摘要

PURPOSE: A method for removing lipopolysaccharides(LPS) from DNA or RNA in a cell is provided, thereby easily removing the lipopolysaccharides from cellular DNA or RNA. CONSTITUTION: A method for removing lipopolysaccharides(LPS) from DNA or RNA in a cell is characterized by using the difference of precipitation between lipopolysaccharides(LPS) and DNA according to the change of alcohol content, wherein lipopolysaccharides(LPS) are removed when the alcohol content is low and bacterial DNA or RNA precipitates are obtained by the addition of alcohol to the supernatant; the alcohol is methanol, ethanol or isopropanol; and the lipopolysaccharides(LPS) are removed by adding the alcohol into a sample of DNA or RNA and centrifuging it at 5,000 to 15,000 rpm and 0 to 30 deg. C for 1 sec to 72 hours.