Measuring DNA polymerising enzyme activity in body fluids, cells or virus infected cultures

摘要

Method for determining the activity of a nucleotide-polymerising enzyme in a sample comprises: (a) capturing the enzyme with a monoclonal antibody (MAb) immobilised on a fixed carrier (the MAb binds the enzyme without significantly affecting its activity); (b) removing impurities and/or disturbance factors; (c) starting nucleotide polymerisation by adding a reaction soln. contg. a primer/template construction and nucleotide substrate; (d) providing reaction conditions to favour permanent association between the enzyme and primer/template prod. complex; (e) washing the nucleotide substrate, primer/template and reaction soln. to remove freshly synthesised polymer; (f) measuring the amt. of nucleotide incorporated with the polymer by standard techniques, and (g) determining enzyme activity.