STABILISATION OF DOUBLE-STRANDED NUCLEIC ACIDS USING PROTEINS
摘要
A composition for treating a nucleic acid duplex, wherein the composition is capable of inhibiting denaturation of the duplex, characterised in that the composition comprises a ubiquitin−like protein and/or a macroglobulin. It has been found that ubiquitin−like proteins (<i>e.g.</i> ubiquitin, NEDD8, RAD23 <i>etc.</i>) and macroglobulins (<i>e.g.</i> &agr;<sb>2</sb>−macroglobulin) are able to stabilise nucleic acid duplexes. A nucleic acid duplex which has been contacted with the composition of the invention can be subjected to more stringent processing conditions, with denaturation of the duplex being inhibited, than would otherwise be possible. Corresponding methods and uses are also provided.
申请公布号
WO03050278(A2)
申请公布日期
2003.06.19
申请号
WO2002GB05617
申请日期
2002.12.11
申请人
WHATMAN INTERNATIONAL LIMITED;PATHIRANA, NAVIN, DEEPAL;BUTT, NEIL;BAKER, MATTHEW
发明人
PATHIRANA, NAVIN, DEEPAL;BUTT, NEIL;BAKER, MATTHEW