LYSOMALE PROTEINE, HERGESTELLT IN DER MILCH VON NICHTMENSCHLICHEN TRANSGENEN TIEREN

摘要

The invention provides transgenic nonhuman mammals producing phosphorylated lysosomal proteins in their milk, and methods of generating the same. Phosphorylation occurs at the 6' position of a mannose side chain residue. Also provided are methods of purifying lysosomal proteins from milk, and incorporating the proteins into pharmaceutical compositions for use in enzyme replacement therapy.