| 摘要 |
<p>Disclosed are methods and compositions for detecting a chromosomal rearrangement in a sample of nucleic acids. In an exemplary method, a first nucleic acid comprising a portion of a first chromosome, which may be detectably labeled, is attached to a substrate; a first portion of a test nucleic acid is hybridized to the first nucleic acid; a second nucleic acid comprising all or a portion of a second chromosome, which may be detectably labeled, is hybridized to a second portion of the test nucleic acid, thereby forming a trimolecular sandwich, and the hybridisation of the test nucleic acid to both of the first and second nucleic acids is detected as an indication that the test nucleic acid comprises a chromosomal rearrangement. In particular embodiments, the first and second nucleic acids are derived from the same chromosome. In a related method, the test nucleic acid is used as a template for nucleic acid synthesis, and primers derived from a first and a second chromosome or from the same chromosome, which are distinctly labeled with first and second labels, respectively, are used to prime nucleic acid synthesis. A synthesized nucleic acid comprising each of the first and second primers is detected as an indication that the test nucleic acid comprises a chromosomal rearrangement. Also disclosed are kits for detecting chromosomal rearrangements. Such methods and kits can be used, for example, in the diagnosis or identification of disease-associated chromosomal rearrangements, e.g., cancers such as leukemia.</p> |
