COMPLEX OF ASSIGNED MOLECULE WITH C-END LABELED PROTEIN, ASSIGNED MOLECULE COMPLEX AND METHODS OF ANALYZING INTERACTION BETWEEN PROTEINS USING THESE COMPLEXES

摘要

A method of analyzing interaction between proteins with the use of an assigned molecule and a C−end labeled protein forming a complex, wherein the complex is formed by the interaction between the genotype/phenotype assigned molecule and the c−end labeled protein having a C−end labeling containing puromycin or its derivative, and part other than protein constituting the assigned molecule and the C−end labeling respectively have modifications required in analyzing the interaction. A method of analyzing interaction between proteins with the use of assigned molecules forming an assigned molecule complex which is a complex containing a plural number of genotype/phenotype assigned molecules and formed by the interaction among these genotype/phenotype assigned molecules and which is characterized by having modifications required in analyzing the interaction in parts other than proteins constituting the assigned molecules.