| 摘要 |
A vector comprising one recognition sequence of a first restriction enzyme of which digestion site exists at a particular position with respect to the recognition sequence and does not exist within the recognition sequence and one recognition sequence of a second restriction enzyme of which digestion site is specific, wherein a fragment obtained by digestion of the vector with the first restriction enzyme can be digested with the second restriction enzyme and a distance between the digestion site of the first restriction enzyme and the digestion site of the second restriction enzyme is 30 nucleotides or shorter, and a method for producing DNA having a target sequence by using the vector.
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