METHOD FOR PREPARING BIOLOGICALLY ACTIVE PROTEIN CONCENTRATE ENRICHED WITH PANCREATIC RIBONUCLEASE A, ANGIOGENIN AND LYSOZYME FROM DAIRY ULTRAFILTRATE

摘要

FIELD: dairy industry. SUBSTANCE: invention relates to isolation of biologically active milk proteins, among them pancreatic ribonuclease A, angiogenin and lysozyme. Ultrafiltrate as a dairy raw is concentrated firstly by 10-12 times by means of reverse osmosis through membrane with pore size 1 000-5 000 Da under pressure 0.4-0.7 MPa and temperature 4-10 C. Then method involves purification of protein solution from lactose and mineral substances by addition of potassium-phosphate buffer, pH is 6.7-7.0, by diafiltration. Prepared protein concentrate is sterilized by microfiltration through membrane with pore size 0.10-0.45 mcm under pressure 0.2-0.7 MPa and temperature 4-10oC. Invention ensures to enhance biological activity of protein concentrate due to enrichment with RNAase, angiogenin and lysozyme preparing from dairy ultrafiltrate using membrane processes. EFFECT: improved preparing method. 1 dwg