摘要 |
It is intended to provide a formate dehydrogenase which has a high specific activity, a small Km values to formic acid and NAD, a temperature-stability over a wide range, a pH-stability over a wide range, a wide function temperature range and a wide function pH range, is not inactivated even in case of coexisting in an enzymatic reduction system of a haloketone compound , and can efficiently regenerate a coenzyme. A formate dehydrogenase-producing strain is searched for from soil and an enzyme having the above-described properties and suitable for industrial use is provided. Moreover, a DNA containing the gene of this enzyme, a recombinant DNA with a vector and its transformant constructed by using a plasmid are provided. Furthermore, a process for producing the formate dehydrogenase using a strain belonging to the genus Thiobacillus or a transformant constructed by using a formate dehydrogenase gene originating in this strain, and a method of regenerating a coenzyme in an enzymatic reduction system with the use of this enzyme are provided.
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