发明名称 |
One step method for micro-production of tea leaves |
摘要 |
The invention provides a one step method for micropropagation of tea plants from leaf explants via callus phase, wherein the explants are maintained in basal Murashige and Skoog medium (Murashige T. and Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473-497; 1962) supplemented with vitamins like thiamine-HCl (0.05 to 2.0 mg/l), pyridoxine-HCl (0.25 to 1.5 mg/l) and nicotinic acid (0.25 to 1.5 mg/l) together with glycine (1.0 to 3.0 mg/l) and either 2.5, 5.0 or 7.5 mg/l 2,4-Dichlorophenoxy acetic acid for a period of 14-16 weeks, from the stage of induction of callus to rhizogenesis and shoot formation.
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申请公布号 |
US6548300(B1) |
申请公布日期 |
2003.04.15 |
申请号 |
US20010811777 |
申请日期 |
2001.03.20 |
申请人 |
COUNCIL OF SCIENTIFIC & INDUSTRIAL RESEARCH |
发明人 |
SANDAL INDRA;BHATTACHARYA AMITA;SHARMA MADHU;AHUJA PARAMVIR SINGH |
分类号 |
A01H4/00;C12N5/02;(IPC1-7):C12N5/00;A01H9/00;A01H11/00 |
主分类号 |
A01H4/00 |
代理机构 |
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主权项 |
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