| 摘要 |
GENE TRANSFERENCE AND USES THEREOF The present invention is directed to the coupling of a fusogenic peptide to the non-viral vector of receptor- mediated gene transference and the addition of a nuclear directing signal to the plasmid DNA, which substantially increases the in vitro and in vivo transference efficiency. The fusogenic and caryophyllenic vector constitutes the new generation of non-viral gene vectors which are superior to the first vectors for guarantying the gene expression contained in the plasmid DNA due to its three characteristics: 1) specific activation of the receptor- mediated endocytosis; 2) opportune rescue of plasmid DNA of the acid endosomes; 3) accurate directing of the plasmid DNA to the cellular nucleus. The invention is implemented neurotensin-SPDP-poly-L-lysine and lactosilated poly-L- lysine for transferring genes to dopaminergic neurons via the rector to neurotensin and to hepatocytes via the receptor to galactose, respectively, but it can be applied to any vector using the receptors endocytic via. The synthesis of the fusogenic and caryophyllenic vector is an easy, rapid, innocuous and economical process in comparison to the technology employed for the fabrication of viral vectors.
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