| 摘要 |
An adenoviral vector comprising an adenoviral genome comprising (i) at least one deletion in a region of the adenoviral genome selected from the group consisting of E1, E2A and E4, (ii) (a) at least one deletion in the VAI gene of the adenoviral genome, alone or in further combination with at least one deletion in the VAII gene of the adenoviral genome, (b) a recombinant VAI gene, alone or in further combination with a recombinant VAII gene, wherein the recombinant gene comprises either of a regulatable promoter in place of the native promoter or a mutated native promoter and 5' to the mutated nativ e promoter, a pol II promoter, or (c) a dominant negative, double-stranded, RN A- dependent protein kinase (PKR) and, optionally, (iii) a polymerase II (pol I I) construct comprising a pol II promoter operably linked to a coding region and/or a polymerase III (pol III) construct comprising a pol III promoter operably linked to a coding region, as well as a system comprising such an adenoviral vector and a cell line that complements the adenoviral vector, an d related systems and methods.
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