METHOD FOR IDENTIFYING MODULATORS OF TRANSCRIPTION

摘要

A method for identifying a modulator of RNA polymerase (RNAP) comprises providing a host cell which expresses a first RNAP and has a first and second polynucleotide construct. The first polynucleotide construct comprises a first promoter operably linked to a first gene wherein the first gene is transcribed by the first RNAP. The second polynucleotide construct comprises a second promoteroperably linked to a second gene which is a reporter gene and wherein the second reporter gene is transcribed by a second RNAP. The host cell is provided with a source of the second RNAP. A test substance is contacted with the host cell under conditions that would permit the expression of the firs and second genes in the absence of the test substance. It can be determined thereby whether the test substance modulates RNAP.