发明名称 |
TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES AND METHODS OF USE |
摘要 |
The present invention describes methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases. The methods provided by this invention utilize a nucleoside polyphosphate, dideoxynucleoside polyphosphate, or deoxynucleoside polyphosphate analogue which has a colorimetric dye, chemiluminescent, or fluorescent moiety, a mass tag or an electrochemical tag attached to the terminal-phosphate. When a nucleic acid polymerase uses this analogue as a substrate, an enzyme-activatable label would be present on the inorganic polyphosphate by-product of phosphoryl transfer. Cleavage of the polyphosphate product of phosphoryl transfer via phosphatase leads to a detectable change in the label attached thereon. When the polymerase assay is performed in the presence of a phosphatase, there is provided a convenient method for real-time monitoring of DNA or RNA synthesis and detection of a target nucleic acid. |
申请公布号 |
WO03020984(A2) |
申请公布日期 |
2003.03.13 |
申请号 |
WO2002US27563 |
申请日期 |
2002.08.29 |
申请人 |
AMERSHAM BIOSCIENCES CORP |
发明人 |
NELSON, JOHN;FULLER, CARL;SOOD, ANUP;KUMAR, SHIV |
分类号 |
C07H19/10;C07H19/20;C07H21/00;C12Q1/42;C12Q1/68 |
主分类号 |
C07H19/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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