| 摘要 |
The invention relates to a recombinant influenza virus for high-yield expression of incorporated foreign gene(s), which is genetically stable in the absence of any helper virus; wherein (a) at least one of the regular viral RNA segments has been exchanged for a vRNA encoding a foreign gene; and/or (b) at least one of the regular viral RNA segments is an ambisense RNA molecule, containing one of the standard viral genes in sense orientation and a foreign, recombinant gene in antisense orientation, or vice versa, in overall convergent arrangement. The present invention further provides a method for the production of said recombinant influenza virus, a method for constructing specific ribozyme-sensitive influenza carrier strains; pharmaceutical compositions comprising said recombinant influenza virus; and the use of said recombinant influenza virus for preparing medicaments for vaccination purposes. |
