EXPRESSION VECTORS USEFUL IN ANIMAL CELLS AND EXPRESSION VECTORS FOR PREPARING HBV SURFACE ANTIGEN

摘要

PURPOSE: Provided are expression vectors useful in animal cells and expression vectors for preparing HBV surface antigens massively, thereby mass-producing HBV surface antigen by low concentration of DHFR inhibitor. CONSTITUTION: The expression vector useful in dhfr¬-animal cells includes the promotor of Cytomegalovirus where an enhancer region is removed, dhfr linked to the promotor, and a replication origin working in a eukaryote. The expression vector useful in dhfr¬-animal cells for mass-production of HBV surface antigens contains the promotor of Cytomegalovirus where an enhancer region is removed, dhfr linked to the promotor, a replication origin working in a eukaryote, HBV surface antigen coding gene and the promotor linked to the HBV surface antigen coding gene. Wherein the eukaryote is a CHO cell.