METHOD FOR PRODUCING ISOLATED CELL CULTURES, CULTURE MEDIUM FOR CULTIVATING CELL CULTURES AND CELL CULTURE

摘要

The invention relates to a method for cultivating cell cultures containing a plurality of cells, whereby said method comprises one or several steps including the expansion of the cell culture and the modification of the cell s of the cell culture in a culture medium. The aim of the invention is to achieve faster and more regular cultivation in terms of the number of cells present in the culture medium. To achieve this, a cell culture is used, wherein the cells are essentially provided in the form of isolated cells and/or agglomerates with weak cell-cell interactions during said method step . Said interactions can be interrupted by an external influence upon the cultu re medium without the majority of cells being damaged when they are transferred from the agglomerate to separate single cells. The expansion and/or modification of the cells preferably takes place in culture conditions which at least partially block the cell receptors responsible for creating cell-ce ll adherences. The culture medium can have a Ca2+ concentration of <= 0.5 mm ol/1 and /or inhibitors which are specific to the receptors of the cell membrane of the cells that are responsible for cell-cell interactions.