发明名称 |
DNA CODING D-HYDANTOIN HAYDROLASE, DNA CODING N- CARBAMYL-D-AMINO ACID HAYDROLASE, RECOMBINANT DNA CONTAINING THE FORMER GENE, CELL TRANSFORMED BY THE RECOMBINANT GENE, METHOD FOR PRODUCING PROTEIN BY USING THE TRANSFORMED CELL, AND METHOD FOR PRODUCING D-AMINO ACID |
摘要 |
PROBLEM TO BE SOLVED: To provide a method for effectively producing a D-amino acid from a 5-substituted hydantoin, by isolating a D-hydantoin hydrolase gene and an N-carbamyl-D-amino acid hydrolase gene relating to competence of transforming the 5-substituted hydantoin into a D-amino acid from microorganisms which have the transformation competence and forming a recombinant which is enhanced in a production amount of an aimed enzyme through enhancement of gene amplification, transcription, and translation activities. SOLUTION: In this method for producing the D-amino acid, a DNA which has a specified base sequence (refer to the specification) and codes a protein having D-hydantoin hydrolase activity and/or another DNA which has another specified base sequence (refer to the specification) and codes another protein having N-carbamyl-D-amino acid hydrolase activity are used. |
申请公布号 |
JP2003024073(A) |
申请公布日期 |
2003.01.28 |
申请号 |
JP20010209712 |
申请日期 |
2001.07.10 |
申请人 |
AJINOMOTO CO INC |
发明人 |
TAKENAKA YASUHIRO;KIRA IKUO;YOKOZEKI KENZO |
分类号 |
C12N15/09;C12N1/21;C12N9/78;C12N9/80;C12N9/86;C12N15/55;C12P13/04;C12P41/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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