发明名称 |
Method for preparing template DNA from processed vegetable food, which is feasible for amplification of DNA region by PCR method |
摘要 |
Disclosed is a method for preparing a template DNA from a processed vegetable food, comprising the steps of: extracting a crude DNA fraction from a processed vegetable food subjected to at least one of a high temperature treatment, a high temperature grinding treatment, a high pressure treatment and a fermentation treatment, and optionally subjected to defatting; and performing a DNA fractionation treatment of the crude DNA fraction by a polyethylene glycol precipitation method and/or a polyacrylamide gel electrophoresis method. Also disclosed is a method for detecting a gene of a plant in a processed vegetable food derived from the plant by a PCR method in which such a template DNA is used. The processed vegetable food may be an oil source seed.
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申请公布号 |
US6492114(B2) |
申请公布日期 |
2002.12.10 |
申请号 |
US20000494438 |
申请日期 |
2000.01.31 |
申请人 |
DIRECTOR OF NATIONAL FOOD RESEARCH INSTITUTE;MINISTRY OF AGRICULTURE, FORESTRY AND FISHERIES |
发明人 |
ARAHIRA MASAOMI;FUKAZAWA CHIKAFUSA |
分类号 |
A23L1/20;C07H21/00;C12N15/09;C12N15/10;C12Q1/68;G01N33/53;G01N33/566;(IPC1-7):C12Q1/68;C12P19/34;C07H21/02;C07H21/04 |
主分类号 |
A23L1/20 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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