发明名称 |
METHOD FOR NUCLEIC ACID DETECTION VIA Q beta REPLICASE |
摘要 |
Disclosed is amplification of a target sequence using the activity of a replicase that quasi-autocatalytically replicates a specific replicase replicable or "replicatable" sequence, while ensuring fidelity thereof by detecting the presence of the amplified target or antitarget sequence rather than the amplified replicase replicable sequence. A method according to the invention for assaying a target nucleic acid comprising hybridizing a set of one or more amplification probes with a nucleic acid sample is provided. A set of two chemiluminescent detection probes each for detecting portions of amplified target sequence along with a dual photomultiplier tube detection system for simultaneous detection of the two chemiluminescent detection probes are also provided for practicing the invention, for use with a target specific set of amplification probes. Kits for practicing the invention are also provided.
|
申请公布号 |
WO0159162(A3) |
申请公布日期 |
2002.12.05 |
申请号 |
WO2001US04244 |
申请日期 |
2001.02.08 |
申请人 |
BAYER CORPORATION |
发明人 |
MORELLO, ANN, M.;JIANG, QINGPING;MONAHAN, JOHN, E.;LAW, SAY-JONG |
分类号 |
C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|