SMALL MOLECULE DESIGN AGAINST DRUG RESISTANT MUTANTS USING DIRECTED EVOLUTION

摘要

The present invention relates to methods for designing new drugs useful against drug-resistant bacterial cells, viruses, mammalian cells and the like. The method involves identifying a target protein of the drug, selecting for drug-resistant variants that have an altered target protein (variant protein) by directed evolution, determining the three dimensional structure of the target and variant proteins and designing a new drug that can be effective against at least one drug-resistant variant. The present invention can be used to predict future mutations that lead to drug resistance and the type of drugs that are effective to combat such resistance.