| 摘要 |
<p>PCT No. PCT/EP97/01809 Sec. 371 Date Dec. 18, 1997 Sec. 102(e) Date Dec. 18, 1997 PCT Filed Apr. 11, 1997 PCT Pub. No. WO97/40366 PCT Pub. Date Oct. 30, 1997A process and a device are described for detecting physical, chemical, biological or biochemical reactions and interactions on biochemically or chemically functionalized specimen carriers in the form of layers or films from the spectral reflection after irradiation of the specimens with light of different wavelengths. In so doing, parameters for the nature of the specimens are determined in an economical and highly accurate manner and with a high degree of parallelism in the measurements. According to the process, the specimens are arranged on a substrate plate with a carrier layer of a carrier plate and are irradiated with light. The following process steps are carried out: temporally resolved illumination of an areal arrangement of specimens to be analyzed by light of different wavelengths from a tunable light source or from a scanning monochromator which is arranged subsequent to a polychromatic light source; b) the imaging of the beam component reflected on at least one boundary surface of each specimen or of the beam components or interferences reflected and interfered, respectively, at boundary surfaces of each specimen which are arranged one behind the other in the direction of light, this imaging being carried out on a spatially resolving areal detector array or a video camera by means of subsequently arranged optical elements; and c) a wavelength-selective detection of the radiation intensities reflected and influenced by the specimens or of the intensities of the imaged interferences, determination of a wavelength spectrum associated with each specimen, and derivation of parameters characterizing the interactions and reactions to be analyzed.</p> |
