NOVEL SOLUBLE ENDOPROTEASES FOR THE <i>IN VITRO</i> PROCESSING OF RECOMBINANT PROTEINS
摘要
The present invention relates to the expression and secretion in <i>Saccharomyces cerevisiae</i> of readily purifiable soluble variants of the Kex1 endopeptidase of <i>Kluyveromyces lactis</i><and the purification and use thereof for the <i>in vitro</i> processing of recombinant proteins usable in industrial applications. The soluble Kex1 endoproteases described here are free from the transmembrane domain of the native enzyme; the deletion of the transmembrane domain is achieved by removing at least 57 amino acid residues from the C-terminal.
