| 摘要 |
A method of conducting accurate identification of biological species with a biochip, and a method of effectuating identification of biological species at a level higher than species are provided. Selection of specific probes for multiple biological species is also facilitated. A plurality of partial sequences A, A'; B, B'; and so on, which are specific to respective targets, are selected as probes in a manner that the partial sequences do not overlap one another. In addition, DNA regions I, J, K and L, which are common to some targets, respectively, are also selected as probes. Alternatively, if there is a common base sequence at leaves below a certain node based on a dendrogram of targets, such a base sequence is designed as a probe unique to the node. By using a set of probes including the probes unique to the targets and the probes unique to leaves, identification of biological species can be performed accurately, and identification of biological species at a level higher than species is also effectuated. In a case where the base sequences corresponding to leaves are identical or similar to each other, such base sequences can be used as probes if sequences corresponding to nodes are different. Therefore, selection of specific probes among multiple biological species can be facilitated.
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