| 摘要 |
<p>A method of investigating the target for a molecule suspected to have inhibitory activity on a biochemical pathway, the method comprising the steps of: Providing further yeast strains which differ from a first yeast strain, which is deficient for one or more genes encoding proteins involved in a yeast biochemical pathway and in.which the deficiency is complemented by the expression of one or more genes encoding proteins of a mammalian biochemical pathway and in which an endpoint indicative of activation of the yeast biochemical pathway can be inhibited by an inhibitory molecule when applied to the first yeast strain under conditions which would normally lead to the activation of yeast biochemical pathway, in that they include mutations in the mammalian gene or one of the mammalian genes; Exposing the further yeast strains to the inhibitory molecule under conditions which would normally lead to the activation of the yeast biochemical pathway; and for each strain, looking for inhibition of the endpoint, Whereby non-inhibition of the endpoint in the presence of the inhibitory molecule indicates that the mutated mammalian gene of that strain is likely to encode the target of the inhibitory molecule.</p> |
