Disclosed is a method of altering a nucleic acid. The method includes fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a criterion.
申请公布号
WO02074978(A2)
申请公布日期
2002.09.26
申请号
WO2002US08376
申请日期
2002.03.19
申请人
PRESIDENT AND FELLOWS OF HARVARD COLLEGE;LIU, DAVID, R.;BITTKER, JOSHUA, A.