METHOD FOR EXTRACTING OLEAGINOUS SUBSTANCES FROM GERMINATION-ACTIVATED GANODERMA LUCIDUM SPORES

摘要

The present invention relates to a method for extracting the oleaginous substances from sporoderm-broken Ganoderma spores using SCF-CO2. The method contains the steps of: (1) inducing germination of Ganoderma spores by incubating the spores in a nutritional solution; (2) activating the Ganoderma spores by placing the germination-induced spores in a well ventilated culture box kept at constant temperature and humidity; (3) breaking the Ganoderma spores by a mechanical means to obtain the sporoderm-broken spores; and (4) extracting the oleaginous substances from the sporoderm-broken spores using a supercritical fluid-carbon dioxide (SCF-CO2) extraction method. The preferred supercritical conditions include 5 M to 60 M Pa of pressure; 32° C. to 85° C. of temperature; and 5 kg/h to 80 kg/h of flow capacity rate. The total extraction time in SCF-CO2 is between 0.5 hour to 6 hour. The method produces approximately 37% by weight of oleaginous substances from the sporoderm-broken Ganoderma spores. These oleaginous substances are transparent and contain the special fragrance of Ganoderma spores. There is no trace of deposit, solvent residue, or oxidization in the oleaginous substances.