| 摘要 |
Methods of detecting carcinoma-associated antigens in patient sera have been discovered. Aspects of the invention utilize single determinant immuno PCR to detect the presence or absence of a tumor associated antigens (e.g., gastric carcinoma-associated antigen MG7-Ag) in human sera. In some embodiments, a biotinylated monoclonal antibody (e.g., MG7-Ab), an avidin linker, and a biotinylated DNA are employed. The methods described herein allow for the early diagnosis of cancers, including, but not limited to, gastric carcinoma and cancers of the liver, colon, breast, uterus, and lung that display a tumor associated antigen. Some embodiments can be used to detect cancers at an early stage, to screen large populations of individuals for various cancers, diagnose the reoccurrence of cancer after surgery, and determine whether an individual suffers from metastasis.
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