发明名称 |
Gene coding for DNA ligase of hyperthermophilic bacteria Aquifex pyrophilus and protein expressed therefrom |
摘要 |
The present invention relates to a gene coding for a thermostable DNA ligase from Aquifex pyrophilus, a hyperthermophilic Bacterium, a protein having an amino acid sequence expressed therefrom and a probe used for detecting said ligase DNA. More particularly, the present invention is directed to a gene having a base sequence of SEQ. ID. NO: 3 and a thermostable DNA ligase having an amino acid sequence expressed therefrom (SEQ. ID. NO: 4).Since Aquifex pyrophilus DNA ligase of the present invention has 75% of the ligation activity after heating at 95 ° C. for 1 hour, the ligase has higher stability than DNA ligases from the preexisting bacteria under high temperature and thus it can be advantageously used in genetic diseases assay of medical field, etc.
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申请公布号 |
US6444429(B1) |
申请公布日期 |
2002.09.03 |
申请号 |
US20000708426 |
申请日期 |
2000.11.09 |
申请人 |
KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY |
发明人 |
HAN YE-SUN;YU YEON-GYU;LIM JAE-HWAN |
分类号 |
C12N15/52;C12N9/00;C12N15/70;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/52 |
代理机构 |
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地址 |
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