HANSENULA POLYMORPHA GENE AND STRAIN LACKING OF THE SAME GENE

摘要

PURPOSE: A Hansenula polymorpha gene and a strain lacking of the same gene are provided, thereby a foreign protein having decreased carboxy terminal digestion can be produced from the strain lacking of the Hansenula polymorpha gene. CONSTITUTION: A gene KEX1 having the nucleotide sequence of SEQ ID NO: 3 and encoding carboxypeptidase alpha derived from Hansenula polymorpha DL1(ATCC 26012). A recombinant vector pKUZ is constructed by inserting Hansenula polymorpha URA3 gene pop out cassette into a plasmid pKH3.9 containing the gene KEX1 having the nucleotide sequence of SEQ ID NO: 3. The Hansenula polymorpha DL1 carboxypeptidase alpha mutant strain is produced by transforming with the recombinant vector pKUZ. A foreign protein is expressed by inserting the foreign gene into the transformed mutant strain and culturing the strain in a methanol medium, wherein the foreign protein is hEGF.