发明名称 |
Regulated autocrine growth of mammalian cells |
摘要 |
Methods of production of desired recombinant proteins, polypeptides and peptides are disclosed which utilize mammalian host cells engineered for autonomous and regulated growth in low cost protein/serum-free culture media. Preferred host cells express insulin or an insulin-like growth factor, and/or transferrin and are engineered so that addition of an inducer (e.g., ZnCl2+CdCl2) halts growth and simultaneously induces the expression of the desired recombinant protein, polypeptide or peptide.
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申请公布号 |
US2002102650(A1) |
申请公布日期 |
2002.08.01 |
申请号 |
US20010964338 |
申请日期 |
2001.09.28 |
申请人 |
HUNT SYBILLE MARIE;GRAY PETER PHILIP;SLEIGH MERILYN JOY |
发明人 |
HUNT SYBILLE MARIE;GRAY PETER PHILIP;SLEIGH MERILYN JOY |
分类号 |
C12N15/09;C12N5/00;C12N5/10;C12N15/63;C12N15/85;C12P21/02;C12R1/91;(IPC1-7):C12P21/02;C12N15/74 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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