摘要 |
A compound represented by the following general formula: <CHEM> wherein R<1> and R<2> represent amino groups present at adjacent positions each other on the phenyl ring; R<3>, R<4>, R<5> and R<6> independently represent a C1-6 alkyl group; R<7>, R<8>, R<9> and R<10> independently represent a hydrogen atom, a C1-6 alkyl group, an allyl group, or a halogen atom; R<11> represents a hydrogen atom or a C1-18 alkyl group; and X<-> represents an anion, and an agent for nitric oxide measurement comprising said compound. The compound efficiently reacts with nitric oxide to give a fluorescent compound that emits strong fluorescence by irradiation with excitation light of a long wavelength. This fluorescent compound is characterized in that said compound is detectable in a fluorescence wavelength range hardly influenced by autofluorescence of cells, and its fluorescence intensity is not attenuated under an acidic condition. |