CORE 1 beta 3-GALACTOSYL TRANSFERASES AND METHODS OF USE THEREOF

摘要

Core 1 beta 3-galactosyl transferases and nucleic acids encoding the core (1) beta 3-galactosyl transferases are described. The enzymes and the nucleic acids encoding said enzymes have been identified in human, rat, mouse D. melanogaster and C. elegans . The polypeptides exhibit a wide range of homologies. The polynucleotides can be used to transform or transfect host cells for producing substantially pure forms of the enzyme, or for use in an expression system for post-translational core (1) O -linked glycosylation of proteins or peptides produced within the expression system. The enzymes can be used to galactosylate, via a beta 3-linkage, an N-acetylgalactosamine linked to a serine, threonine or other O -linking amino acid on peptides or proteins requiring O -linked glycosylation.