ENRICHMENT AND DETECTION OF NUCLEIC ACIDS WITH ULTRA-HIGH SENSITIVITY

摘要

Methods, systems, and devices are disclosed for enrichment and detection of molecules of a target biomarker. In one aspect, In one aspect, a biosensor device for enriching and detecting biomarker molecules include a substrate, and a microarray of hydrophilic islands disposed on the substrate. A sensing area on each of the microarray hydrophilic islands is structured to anchor bio-molecular probes of at least one type for detecting molecules of a target biomarker and to attract an array of nanodroplets of a biomarker solution that includes the target biomarker molecules. A hydrophobic surface disposed to surround the microarray of hydrophilic islands.

主权项

1. A biosensor device for enriching and detecting biomarker molecules, the biosensor device comprising:
a substrate; a microarray of hydrophilic islands disposed on the substrate; sensing areas disposed on the microarray hydrophilic islands, the sensing areas structured to anchor bio-molecular probes of at least one type for detecting molecules of a target biomarker and to attract an array of droplets of a biomarker solution that includes the target biomarker molecules; and a hydrophobic surface surrounding the microarray of hydrophilic islands, the hydrophobic surface including nanostructures having rough surfaces to enrich the target biomarker molecules on the sensing area by enhancing evaporation of the array of droplets leading to an enriched array of droplets with an increased concentration of the target biomarker molecules compared to before evaporation; wherein the sensing areas are structured to receive a layer of water-immiscible-liquid over the enriched array of droplets to form water-immiscible-liquid encapsulated reaction chambers for controlling a reaction between the target biomarker molecules and the bio-molecular probes.