发明名称 |
Method of producing a biosensor protein capable of regulating a fluorescence property of the modified green fluorescent protein therein, and the biosensor protein produced by the method |
摘要 |
<p>A biosensor protein comprising (1) and (2) below: (1) a modified fluorescent protein which is obtained by cleaving amino acid sequence of green fluorescent protein or its derivative in the vicinity of a hotspot amino acid residue which affects the fluorescence property, and modifying the structure of the green fluorescent protein or its derivative, and (2) one or more functional molecules which are capable of transmitting their conformational changes to the modified fluorescent protein to cause a conformational change of the modified fluorescent protein, thereby altering the fluorescence property of the modified fluorescent protein. <IMAGE> <IMAGE> <IMAGE> <IMAGE></p> |
申请公布号 |
EP1209167(A1) |
申请公布日期 |
2002.05.29 |
申请号 |
EP20010127775 |
申请日期 |
2001.11.21 |
申请人 |
OKAZAKI NATIONAL RESEARCH INSTITUTES |
发明人 |
NAKAI, JUNICHI |
分类号 |
G01N33/53;C07K5/062;C07K5/083;C07K7/06;C07K14/435;C07K14/47;C07K19/00;C12N5/02;C12N9/12;C12N15/09;C12P21/02;C12R1/91;G01N21/78;G01N33/566;(IPC1-7):C07K14/435;C12N15/62;G01N33/50 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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