METHODS FOR LARGE SCALE PRODUCTION OF RECOMBINANT DNA-DERIVED TPA OR K2S MOLECULES
摘要
The invention belongs to the field of thrombolysis and of tissue plasminogen activator (tPA) derivative production in prokaryotic cells. The invention relates to methods for the production of a recombinant DNA-derived tPA, a variant thereof or a (Kringle 2 Serine) K2S molecule or a variant thereof in prokaryotic cells, wherein said tPA or K2S or variant is secreted extracellularly as an active and correctly folded protein, and the prokaryotic cell contains and expresses a vector comprising the DNA coding for said tPA or K2S or variant operably linked to the DNA coding for the signal peptide OmpA. The invention further relates to specific K2S derivatives obtainable by said method. The invention further relates to said DNA molecules and the use of said DNA molecules in said methods.
申请公布号
WO0240650(A2)
申请公布日期
2002.05.23
申请号
WO2001EP12857
申请日期
2001.11.07
申请人
BOEHRINGER INGELHEIM INTERNATIONAL GMBH;WERNER, ROLF-GUENTHER;GOETZ, FRIEDRICH;TAYAPIWATANA, CHATCHAI;MANOSROI, JIRADEJ;MANOSROI, ARANYA