发明名称 |
Acoustic standing-wave enhancement of a fiber-optic salmonella biosensor |
摘要 |
A fluorescent fiber-optic biosensor system using ultrasonic concentration of particles and cells for the detection of Salmonella typhimurium. A biosensor test chamber serves as an ultrasonic standing-wave cell that allows microspheres or cells to be concentrated in parallel layers or in a column along the axis of the cell. A fiber probe along the axis delivers laser excitation to fluorescent-labeled antibodies of Salmonella and collects the fluorescent signal. The Salmonella-antibody complexes are moved acoustically to the axis of the cell, increasing the fluorescent signal. Alternatively, the Salmonella-labelled antibody complexes attach to unlabeled antibodies that have been immobilized on the surface of polystyrene microspheres. This entire structure can be manipulated acoustically and the increase in the fluorescent signal, which can be an order of magnitude, indicates the presence of Salmonella.
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申请公布号 |
US6391653(B1) |
申请公布日期 |
2002.05.21 |
申请号 |
US20000619230 |
申请日期 |
2000.07.19 |
申请人 |
THE BOARD OF GOVERNORS FOR HIGHER EDUCATION, STATE OF RHODE ISLAND AND PROVIDENCE PLANTATIONS |
发明人 |
LETCHER STEPHEN V.;RAND A. GARTH;ZHOU CHONGHUA |
分类号 |
G01N33/543;G01N33/569;(IPC1-7):G01N33/543 |
主分类号 |
G01N33/543 |
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