A METHOD TO DETERMINE AN ENGRAFTING CELL DOSE OF HEMATOPOIETIC STEM CELLTRANSPLANT UNITS

摘要

A method to determine an engrafting cell dose of hematopoietic stem cell transplant units from transplant sources having nucleated cells selected from the group consisting of cord blood, bone marrow, peripheral blood comprising the steps of - subjecting the source to a substantially complete erythrocyte lysis, - measuring in a cell counter a signal corresponding selectively to white blood cells, - assessing essentially quantitatively nucleated red blood cell (NRBC) count as part of the total nucleated cell (NC) count, - and determining the number of white blood cells (WBCs) as transplant relevant cells.