| 摘要 |
A synthetic transport entity is used for transferring a nucleic acid sequence of interest across a biological membrane. The transport entity comprises a functional element (FE), which increases the efficiency of transfection (e.g. polyethylene glycol (PEG) or a nuclear localisation signal), a binding element (BE), such as a peptide nucleic acid (PNA), and a carrier molecule comprising the nucleic acid of interest and a BE target sequence. The transport entity can be altered in a controlled manner at the surface of the membrane or after having passed across the membrane or having been taken up by the cell, and comprises at least one alteration site that, when altered, changes a property of the transport entity. |
