发明名称 |
MICROBIOLOGICAL PRODUCTION METHOD FOR alpha-L-ASPARTYL-L-PHENYLALANINE. |
摘要 |
The present invention relates to a new, microbiological method for the production of alpha-L-aspartyl-L-phenylalanine (Asp-Phe) from the substrates L-aspartic acid (L-Asp) and L-phenylalanine (L-Phe) wherein the substrates are contacted, in the presence of ATP, with a non-ribosomal dipeptide synthetase comprising two minimal modules connected by one condensation domain wherein the N- resp. C-terminal modules are recognising L-Asp and L-Phe, respectively, and the latter module is covalently bound at its N- terminal end to the condensation domain, and wherein each of these minimal modules is composed of an adenylation domain and a 4'-phosphopantetheinyl cofactor containing thiolation domain, and that the Asp-Phe formed is recovered. The present invention also relates to novel DNA fragments or combination of DNA fragments encoding a new Asp-Phe dipeptide synthetase, micro-organisms containing such DNA fragments, as well as to the new Asp-Phe dipeptide synthetases itself. |
申请公布号 |
MXPA01009875(A) |
申请公布日期 |
2002.04.24 |
申请号 |
MX2001PA09875 |
申请日期 |
2000.03.28 |
申请人 |
HOLLAND SWEETENER COMPANY V.O.F. |
发明人 |
QUAEDFLIEG, PETER, JAN, LEONARD, MARIO |
分类号 |
C12N15/09;C07K5/06;C12N1/21;C12N9/00;C12N15/52;C12N15/62;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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