Process for the fermentative preparation of D-pantothenic acid using coryneform bacteria

摘要

The invention provides a process for preparing D-pantothenic acid by the fermentation of coryneform bacteria in which bacteria are used in which the nucleotide sequence (pck gene) coding for phosphoenolpyruvate carboxykinase (EC 4.1.1.49) is attenuated, wherein the following steps are performed: a) fermentation of D-pantothenic acid producing bacteria in which at least the gene coding for phosphoenolpyruvate carboxykinase is attenuated, b) enrichment of D-pantothenic acid in the medium or in the cells of the bacteria, and c) isolation of the D-pantothenic acid produced.