METHOD AND DEVICE FOR DETECTING FLUORESCENCE IN CONFOCAL SCANNING MICROSCOPIC METHOD

摘要

PROBLEM TO BE SOLVED: To provide a method and a device for optimizing or increasing the fluorescent light photon collection quantity of a fluorescent substance excited by multiple photon excitation so as to generate fluorescence in order to detect an optimum sample as to a method and a device for detecting fluorescence in a scanning microscopic method, especially a confocal scanning microscopic method. SOLUTION: The operation variable of a light source (2) generating multiple photon excitation and/or the system variable of a confocal scanning microscope is suited to the characteristics of a fluorescent material as to the optimum fluorescent photon collection quantity.