发明名称 |
Process for the preparation of endotoxin-free or endotoxin-depleted nucleic acids and/or oligonucleotides for gene therapy |
摘要 |
A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein said nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in that said essentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of said biological sources by per se known mechanical methods, such as centrifugation, filtration; the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed by isolation of said nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.
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申请公布号 |
US2002032324(A1) |
申请公布日期 |
2002.03.14 |
申请号 |
US20010962459 |
申请日期 |
2001.09.26 |
申请人 |
QIAGEN GMBH |
发明人 |
COLPAN METIN;SCHORR JOACHIM;MORITZ PETER |
分类号 |
B01J20/281;A61K31/70;A61K31/7088;A61K48/00;A61P35/00;A61P43/00;B01J20/26;B01J41/04;C07H1/08;C07H21/00;C07H21/02;C07H21/04;C12N15/10;C12N15/87;G01N30/88;(IPC1-7):C07H21/04;C12N1/08 |
主分类号 |
B01J20/281 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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