| 摘要 |
<p>The present invention is related to a method for the recovering and the characterization of a surface antigenic structure of a trypanosome flagellar pocket (fp), characterized in that it comprises the following steps:- purifying membrane glycoproteins located on the surface of the flagellar pocket (fp) of said trypanosome, preferably glycoproteins containing linear poly-N-acetyllactosamine (pNAL), from a biological extract comprising said trypanosome,- deglycosylating said glycoproteins into proteins,- obtaining hybridomas secreting monoclonal antibodies directed against said proteins, - screening the resulting hybridomas for selecting a positive hybridoma which secretes an antibody able to inhibit the trypanosome growth (trypanosome activity in vitro), - obtaining said antibody from said positive hybridoma, - selecting and purifying an antigenic structure among the proteins of the trypanosome flagellar pocket (fp) by binding said proteins to said monoclonal antibody, and- possibly identifying the amino acid sequence of said antigenic structure and possibly the nucleotide sequence encoding said antigenic structure.</p> |
