| 摘要 |
Histamine may be quantitatively measured by providing the steps of: holding in a recess formed at the bottom of a vessel an oocyte that expresses histamine receptors, inserting first and second electrodes into the oocyte, measuring the membrane potential of the oocyte by means of said first electrode to stabilize the membrane potential of said oocyte at a predetermined level by driving a current through second electrode inserted into said oocyte by means of a circuitry for clamping membrane potential of oocyte, infusing a sample into a fine reacting tube having an antigen immobilized on the inner surface thereof together with some buffer solution to promote a histamine releasing reaction, transferring the solution containing histamine released in the fine reacting tube to the vessel to make contact with said oocyte in the vessel, detecting an electric response of oocyte caused by the contact with said solution, and determining the concentration of histamine released by said histamine releasing reaction in said fine reacting tube. The whole blood or mast cell suspension may be used as a sample without pretreatment.
|
