| 摘要 |
The present invention provides methods and compositions for the production of high titer, wild-type-free recombinant AAV ("rAAV") virions. The compositions of the present invention include novel nucleic acids encoding replication competent but packaging defectrive AAV helper functions and AAV helper function vectors. The present invention also includes host cells transfected by the claimed nucleic acids, methods of using the claimed vectors, and rAAV virions produced by such methods. The similar strategy can also be used for autonomous parvorirus virion production. |
