发明名称 |
METHODS AND COMPOSITIONS FOR TRANSFORMING CELLS USING SITE-SPECIFIC RECOMBINATION |
摘要 |
Methods and compositions for transforming cells, resulting in efficient and stable site-specific integration of transgenes, are disclosed. Transformation is achieved by providing an acceptor DNA containing two inverted lox sequences and a donor DNA containing the same two inverted lox sequences, and then contacting the acceptor and donor DNA with a recombinase (e.g., Cre or Flp) which causes recombination at the lox sequences contained in the DNAs. Prior to recombination, the acceptor DNA is preferably integrated into the genome of a cell, such as an embryonic stem cell or a fertilized egg. The acceptor DNA optionally may further contain a negatively selectable marker to allow for screening of cells which have undergone the desired site-specific recombination (e.g., DNA cassette exchange).
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申请公布号 |
WO0063410(A9) |
申请公布日期 |
2002.01.31 |
申请号 |
WO2000US09782 |
申请日期 |
2000.04.12 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY;ALBERT EINSTEIN COLLEGE OF MEDICINE OF YESHIVA UNIVERSITY;BOUHASSIRA, ERIC;LEBOULCH, PHILIPPE |
发明人 |
BOUHASSIRA, ERIC;LEBOULCH, PHILIPPE |
分类号 |
C12N15/09;C07K14/805;C12N5/10;C12N15/12;C12N15/64;C12N15/867;C12N15/90;(IPC1-7):C12N15/90 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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