Preparing fine chemicals, particularly Vitamins E and K, useful as antioxidants e.g. in foods or medicine, by growing organisms with altered shikimate biosynthesis pathway

摘要

Preparation of fine chemicals (I) by culturing organisms in which the shikimate pathway (SP) has been altered relative to the wild type. Independent claims are also included for the following: (a) nucleic acid construct (A1) containing a sequence that encodes (i) a plastid transit peptide (PTP); and (ii) a 265 amino acid (aa) sequence (4), reproduced, or its derivatives (formed by substitution, insertion or deletion of aa) or sequences with at least 30% homology at the aa level and having chorismate mutase (CM) activity; (b) nucleic acid construct (A2) containing a sequence that encodes CM and/or prephenate dehydrogenase (PH), linked to at least one regulatory sequence for transcription or translation in plants; (c) genetically modified organisms (B) in which metabolite flow in SP, and thus content of (I), is altered relative to the wild type; and (d) method for preparing (B).