发明名称 |
Profiling of protease specificity using combinatorial fluorogenic substrate libraries |
摘要 |
A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of enzymes, such as proteases. The substrates contain a fluorogenic-leaving group, such as 7-amino-4-carbamoylmethyl-coumarin (ACC). Substrates incorporating the ACC leaving group show comparable kinetic profiles as those with the traditionally used 7-amino-4-methyl-coumarin (AMC) leaving group. The bifunctional nature of ACC allows for the efficient production of single substrates and substrate libraries using solid-phase synthesis techniques. |
申请公布号 |
AU6509201(A) |
申请公布日期 |
2001.12.17 |
申请号 |
AU20010065092 |
申请日期 |
2001.05.25 |
申请人 |
REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
JENNIFER L. HARRIS;BRADLEY J. BACKES;JONATHAN A ELLMAN;CHARLES S CRAIK |
分类号 |
C07K1/04;C07K5/103;C07K5/117;C12Q1/37 |
主分类号 |
C07K1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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