HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY

摘要

The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.